Recombinant human MSLN (NP_005814.2) (Glu296-Gly580) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
The recombinant human MSLN consists 287 amino acids and predicts a molecular mass of 32.4 kDa. The apparent molecular mass of the protein is higher than the theoretical value in SDS-PAGE under reducing conditions due to glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method.
>(5.6+73.7+8.4)% as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.