Recombinant human MSR1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
MSR1, as known as macrophage scavenger receptor types I and II isoform type 1, is single-pass type II membrane protein. Scavenger receptors are a group of membrane receptors that recognize and internalize modified low density lipoproteins (LDLs) and participate in the removal of many foreign substances and waste materials in the living body. Scavenger receptors are categorized into three classes, and class A mainly expressed in macrophage includes three isoforms derived by alternative splicing. This protein, has been suggested to play important roles in macrophage growth, cell adhesion, osteoclast differentiation, and as well as intracellular signaling.
In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
42.4kDa (384aa)40-57KDa (SDS–PAGE under reducing conditions.)
ADPKWETKNC SVSSTNANDI TQSLTGKGND SEEEMRFQEV FMEHMSNMEK RIQHILDMEA NLMDTEHFQN FSMTTDQRFN DILLQLSTLF SSVQGHGNAI DEISKSLISL NTTLLDLQLN IENLNGKIQE NTFKQQEEIS KLEERVYNVS AEIMAMKEEQ VHLEQEIKGE VKVLNNITND LRLKDWEHSQ TLRNITLIQG PPGPPGEKGD RGPTGESGPR GFPGPIGPPG LKGDRGAIGF PGSRGLPGYA GRPGNSGPKG QKGEKGSGNT LTPFTKVRLV GGSGPHEGRV EILHSGQWGT ICDDRWEVRV GQVVCRSLGY PGVQAVHKAA HFGQGTGPIW LNEVFCFGRE SSIEECKIRQ WGTRACSHSE DAGVTCTLHH HHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>95% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)