Active Recombinant Human MTOR/RPTOR/MLST8 protein, FLAG/His-tagged

• Cat.No.: MTOR-42H
• Product Overview: Recombinant Human MTOR(1362–end)/RPTOR(2–326 aa)/MLST8(2–1335 aa) fused with N-terminal FLAG-tag (mTOR), N-terminal His-tag (MLST8), N-terminal His-tag (Raptor) was expressed in Insect cells.

Specification

• Description: Complex of human mTOR, also known as FRAP1 (GenBank Accession No. NM_004958), a.a. 1362-end, with N-terminal FLAG-tag, MW = 137 kDa, human MLST8 (GenBank Accession No. NM_022372), a.a. 2-326 (end) with N-terminal His-tag, MW = 37 kDa, and human Raptor (GenBank Accession No. NM_020761), a.a. 2-1335 (end) with N-terminal Histag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.
• Source: Insect cells
• Species: Human
• Tag: FLAG/His
• Form: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, and 0.18 mg/ml FLAG peptide
• Bio-activity: 7.1 pmol/min/µg
• Molecular Mass: 324 kDa
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at -80 centigrade. Avoid freeze/thaw cycles.
• Concentration: 0.27 mg/ml

Gene Information

• Gene Name: MTOR mechanistic target of rapamycin (serine/threonine kinase) [ Homo sapiens ]
• Official Symbol: MTOR
• Synonyms: MTOR; mechanistic target of rapamycin (serine/threonine kinase); FK506 binding protein 12 rapamycin associated protein 1 , FRAP, FRAP1, FRAP2; serine/threonine-protein kinase mTOR; dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1); FK506 binding protein 12 rapamycin associated protein 2
• Gene ID: 2475
• mRNA Refseq: NM_004958
• Protein Refseq: NP_004949
• MIM: 601231
• UniProt ID: P42345
• Chromosome Location: 1p36
• Pathway: AMPK signaling, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem
• Function: ATP binding; RNA polymerase III type 1 promoter DNA binding; RNA polymerase III type 2 promoter DNA binding; RNA polymerase III type 3 promoter DNA binding; TFIIIC-class transcription factor binding; kinase activity; kinase activity; nucleotide binding