Recombinant Human NCL protein, GST-tagged

• Cat.No.: NCL-08H
• Product Overview: Recombinant Human NCL(1 a.a. - 710 a.a.) fused with GST tag at N-terminal was expressed in Wheat Germ.

Specification

• Description: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Molecular Mass: 103 kDa
• AA Sequence: MVKLAKAGKNQGDPKKMAPPPKEVE EDSEDEEMSEDEEDDSSGEEVVIPQ KKGKKAAATSAKKVVVSPTKKVAVA TPAKKAAVTPGKKAAATPAKKTVTP AKAVTTPGKKGATPGKALVATPGKK GAAIPAKGAKNGKNAKKEDSDEEED DDSEEDEEDDEDEDEDEDEIEPAAM KAAAAAPASEDEDDEDDEDDEDDDD DEEDDSEEEAMETTPAKGKKAAKVV PVKAKNVAEDEDEEEDDEDEDDDDD EDDEDDDDEDDEEEEEEEEEEPVKE APGKRKKEMAKQKAAPEAKKQKVEG TEPTTAFNLFVGNLNFNKSAPELKT GISDVFAKNDLAVVDVRIGMTRKFG YVDFESAEDLEKALELTGLKVFGNE IKLEKPKGKDSKKERDARTLLAKNL PYKVTQDELKEVFEDAAEIRLVSKD GKSKGIAYIEFKTEADAEKTFEEKQ GTEIDGRSISLYYTGEKGQNQDYRG GKNSTWSGESKTLVLSNLSYSATEE TLQEVFEKATFIKVPQNQNGKSKGY AFIEFASFEDAKEALNSCNKREIEG RAIRLELQGPRGSPNARSQPSKTLF VKGLSEDTTEETLKESFDGSVRARI VTDRETGSSKGFGFVDFNSEEDAKA AKEAMEDGEIDGNKVTLDWAKPKGE GGFGGRGGGRGGFGGRGGGRGGRGG FGGRGRGGFGGRGGFRGGRGGGGDH KPQGKKTKFE
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: NCL nucleolin [ Homo sapiens ]
• Official Symbol: NCL
• Synonyms: NCL; nucleolin; C23; FLJ45706; FLJ59041
• Gene ID: 4691
• mRNA Refseq: NM_005381
• Protein Refseq: NP_005372
• MIM: 164035
• UniProt ID: P19338
• Chromosome Location: 2q12-qter
• Pathway: Aurora B signaling, organism-specific biosystem; Pathogenic Escherichia coli infection, organism-specific biosystem; Pathogenic Escherichia coli infection, conserved biosystem; Regulation of Telomerase, organism-specific biosystem; T Cell Receptor Signaling Pathway, organism-specific biosystem; Validated targets of C-MYC transcriptional activation, organism-specific biosystem
• Function: DNA binding; RNA binding; nucleic acid binding; nucleotide binding; protein C-terminus binding; protein binding; telomeric DNA binding