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Recombinant Human NRAS Protein, His-tagged

Cat.No.: NRAS-447H
Product Overview: Recombinant mature form of human NRAS (P01111) (Met1-Cys186) was expressed with a polyhistidine tag at the N-terminus.
Source: E. coli
Species: Human
Tag: His
Predicted N Terminal: His
Form: Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10% Glycerol, pH 7.5, 5%~8% trehalose and mannitol.
Molecular Mass: The recombinant human NRAS consists of 202 amino acids and predicts a molecular mass of 23 KDa. It migrates as an approximately 24 KDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: NRAS neuroblastoma RAS viral (v-ras) oncogene homolog [ Homo sapiens ]
Official Symbol: NRAS
Synonyms: NRAS; neuroblastoma RAS viral (v-ras) oncogene homolog; GTPase NRas; N ras; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog; NS6; ALPS4; N-ras; NRAS1;
Gene ID: 4893
mRNA Refseq: NM_002524
Protein Refseq: NP_002515
MIM: 164790
UniProt ID: P01111
Chromosome Location: 1p13.2
Pathway: ARMS-mediated activation, organism-specific biosystem; Activation of RAS in B Cells, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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