Recombinant Human NSD2(959-1365 aa)(Catalytic Domain) fused with GST tag at N-terminal was expressed in E. coli.
NSD2/MMSET is a SET-domain containing histone methyltransferase, catalyzing the dimethylation of histone H3 at lysine 36. NSD2/MMSET, an oncogene that is overexpressed in several cancers, is thought to drive pathogenesis of t(4;14) positive multiple myeloma.
In 25 mM Tris pH 8.0, 150 mM NaCl, 1 mM DTT, 1 mM EDTA and 20% glycerol.
NSD2 / MMSET Catalytic Domain, Recombinant Human is useful for histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 0.5 - 2 µg NSD2 per reaction with HeLa or recombinant nucleosomes as a substrate is recommended.
Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.