Recombinant Human PALM(1-387aa) fused with His tag at N-terminal was expressed in E. coli.
PALM is a member of the paralemmin protein family. The product of this gene is a prenylated and palmitoylated phosphoprotein that associates with the cytoplasmic face of plasma membranes and is implicated in plasma membrane dynamics in neurons and other cell types. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
44.5 kDa (410aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
> 85% by SDS - PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.5 mg/ml (determined by Bradford assay)