Active Recombinant Human PCSK9 protein, Avi-His-tagged, Biotinylated
Cat.No. : | PCSK9-187H |
Product Overview : | Biotinylated Recombinant Human PCSK9 protein(Gln 31 - Gln 692(D374Y)), fused with Avi and His tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | Avi-His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : | Immobilized Human LDL R, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human PCSK9 (D374Y), Avitag,His Tag with a linear range of 0.2-8 ng/mL. |
Molecular Mass : | This protein carries an Avi tag (Avitag) at the C-terminus, followed by a polyhistidine tag. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 59.9 kDa respectively. The protein migrates as 17 kDa and 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The D374Y mutation results in higher affinity of PCSK9 for LDLR. |
Protein Length : | Gln 31 - Gln 692(D374Y) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | PCSK9 proprotein convertase subtilisin/kexin type 9 [ Homo sapiens ] |
Official Symbol : | PCSK9 |
Synonyms : | PCSK9; proprotein convertase subtilisin/kexin type 9; HCHOLA3, hypercholesterolemia, autosomal dominant 3; FH3; NARC 1; NARC1; NARC-1; HCHOLA3; |
Gene ID : | 353175 |
MIM : | 607786 |
UniProt ID : | Q8NBP7 |
Products Types
◆ Recombinant Protein | ||
PCSK9-352H | Active Recombinant Human PCSK9 Protein, His & Avi-tagged, Biotinylated | +Inquiry |
PCSK9-1622H | Recombinant Human PCSK9 Protein, His (Fc)-Avi-tagged | +Inquiry |
PCSK9-3273H | Recombinant Human PCSK9 protein(Met1-Gln692), His-tagged, Biotinylated | +Inquiry |
Pcsk9-020M | Recombinant Mouse Pcsk9 Protein, MYC/DDK-tagged | +Inquiry |
Pcsk9-209M | Active Recombinant Mouse Pcsk9 Protein, His-Avi-tagged, Biotinylated | +Inquiry |
◆ Lysates | ||
PCSK9-2306RCL | Recombinant Rat PCSK9 cell lysate | +Inquiry |
PCSK9-2564MCL | Recombinant Mouse PCSK9 cell lysate | +Inquiry |
PCSK9-2775RCL | Recombinant Rhesus PCSK9 cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-1806 | PCSK9(D374T)-LDLR TR-FRET Assay Kit | +Inquiry |
Kit-1808 | PCSK9-LDLR TR-FRET Assay Kit | +Inquiry |
Kit-1807 | PCSK9[Biotinylated]-LDLR Binding Assay Kit | +Inquiry |
Kit-0666 | PCSK9-LDLR in vitro Binding Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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