Recombinant Human PDE4D(2–507 aa) fused with GST tag at N-terminal was expressed in Insect cells.
This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol and 16 mM Glutathione.
≥ 13,600 pmol/min/µg
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37 centigrade.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
>6 months at -80 centigrade. Avoid freeze/thaw cycles.