Recombinant Human PDGFD was expressed in HEK293.
PDGF-DD, also known as platelet-derived growth factor D, IEGF and SCDGFB, is asecreted growth factor belonging to the PDGF/VEGFfamily. It is highly expressed in the heart, pancreas, adrenal glands and ovary. PDGF-DD forms functional homodimers that bind and induce PDGF Rβ homodimers and PDGF Rα/β heterodimers that promote intracellular signaling. This plays an important role in the regulation of cell differentiation, migration and survival. It has also been reported that PDGF-DD can induce monocyte and macrophage recruitment, increase interstitial pressure and facilitate wound healing.
Lyophilized after extensive dialysis against PBS.
ED50 < 5 μg/ml, measured in a cell proliferation assay using 3T3 cells.
19-21 kDa, observed by reducing SDS-PAGE.
SYHDRKSKVD LDRLNDDAKR YSCTPRNYSV NIREELKLANVVFFPRCLLV QRCGGNCGCG TVNWRSCTCN SGKTVKKYHEVLQFEPGHIK RRGRAKTMAL VDIQLDHHER CDCICSSRPPR
< 0.2 EU/μg, determined by LAL method.
> 95% as analyzed by SDS-PAGE.
Lyophilized recombinant human PDGF-DD remains stable up to 6 months at -80 centigradefrom date of receipt. Upon reconstitution, human PDGF-DDshould be stable up to 1 week at 4 centigradeor up to 2 months at -20°C.
Reconstituted in ddH2O or PBS at 100 μg/ml.