Active Recombinant Human PGA4 Protein, His-tagged

• Cat.No.: PGA4-479H
• Product Overview: Recombinant human PGA4 (P00790) (Met 1-Ala 388) was fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Ile 16
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >10,000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant human PGA4 consists of 384 amino acids and has a predicted molecular mass of 41.7 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >97 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PGA4 pepsinogen 4, group I (pepsinogen A) [ Homo sapiens ]
• Official Symbol: PGA4
• Synonyms: pepsinogen A4; pepsinogen-4
• Gene ID: 643847
• mRNA Refseq: NM_001079808
• Protein Refseq: NP_001073276
• MIM: 169720
• UniProt ID: P0DJD7
• Chromosome Location: 11q12.2
• Pathway: Metabolism of proteins, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; Surfactant metabolism, organism-specific biosystem