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Home > Products > Recombinant Proteins > Recombinant Human PI3 kinase (p110α/p85α) protein, GST-tagged

Recombinant Human PI3 kinase (p110α/p85α) protein, GST-tagged

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Flag(1)
GST(3)
His(15)
N/A(1)
Cat.No.:
PIK3CA-1894H
Product Overview:
Recombinant Human PI3 kinase (p110α/p85α) fused with N-terminal GST-tag(p110α), no tag on p85α, was co-expressed in Insect cells.
Source:
Insect cells
Species:
Human
Tag:
GST
Form:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT.
Bio-activity:
880 pmol/min/μg
Molecular Mass:
235 kDa (complex); p110α MW=151 kDa, p85α MW=84 kDa.
Unit Definition:
A unit of p110α/p85α kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37 centigrade.
Applications:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage:
>6 months at –80 centigrade. Avoid freeze/thaw cycles


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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