Recombinant Human PI3 kinase (p110α/p85α) fused with N-terminal GST-tag(p110α), no tag on p85α, was co-expressed in Insect cells.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT.
235 kDa (complex); p110α MW=151 kDa, p85α MW=84 kDa.
A unit of p110α/p85α kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37 centigrade.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
>6 months at –80 centigrade. Avoid freeze/thaw cycles