||Recombinant human POU5F1 fused with C-terminal tag of 11 arginine domain, which efficiently delivery protein intracellularly was expressed in E. coli. To further increase Oct4 activity for gene transcription activation, HSV VP16 TAD (transcription activat
||1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
||DNA binding assay: DNA binding activity was demonstrated using Oct4 specific DNA binding oligo derived EMSA.
||MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAY CGPQVGVGLVPQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFA KLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICKAET LVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNRRQKGKRSSSDYAQREDFEA AGSPFSGGPVSFLLAPGPHFGTPGYGSPHFTALYSSVPFPEGEAFPPVSVTTLGSPMHSNTSRSEPSGPISTGNP SPPSKESHKSPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMF TDALGIDEYGGLEESGGGGSPGRRRRRRRRRRR
||>90% by SDS-PAGE
||1. Protein transduction for protein derived iPS generation.2. Active recombinant protein, may be used for ELISA based DNA/Protein binding assay.3. As specific protein substrate for kinase assay.
||Keep at -20centigrade for long term storage. Product is stable at 4 centigrade for at least 7 days.