Recombinant Human PPM1L, fused with N-His tag, was expressed in E. coli.
PPM1L, or PP2CE, belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. PP2C sregulate stress-activated protein kinase (SAPK; see MIM 601158) signaling cascades that respond to extra cellularstimuli.
20mM Tris.Cl, 50mM NaCl, 50% Glycerol, pH8.0.
22.2kDa(including fusion tag)
196aa(including fusion tag)
WB, ELISA, IP, antibody production, protein array
Store at -80centigrade and avoid repeated freeze-thaw cycles.