PRKAG1 & PRKAB1 & PRKAA1-1237H
Recombinant human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; Recombinant human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2; Recombinant human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tag
Predicted N Terminal:
Met & Met & His
Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.4.
The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 38, 40 & 95 KDa respectively in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>94 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.