PRKAG1 & PRKAB1 & PRKAA2-1240H
Recombinant human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; Recombinant human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2; Recombinant human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagge
Predicted N Terminal:
Met & Met & His
Supplied as sterile 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% gly, 1mM GSH, pH 7.4.
The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 40, 42 & 95 KDa respectively in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>94 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.