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Active Recombinant Human PRKAG1 & PRKAB2 & PRKAA1 Protein, His-GST-tagged

Cat.No. : PRKAG1 & PRKAB2 & PRKAA1-1238H
Product Overview : Recombinant human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; Recombinant human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; Recombinant human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tag
  • Specification
  • Gene Information
  • Related Products
Source : Baculovirus-Insect Cells
Species : Human
Tag : His-GST
Predicted N Terminal : Met & Met & His
Form : Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.4.
Bio-activity : The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Molecular Mass : The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >94 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name : PRKAG1 protein kinase, AMP-activated, gamma 1 non-catalytic subunit [ Homo sapiens ]
Official Symbol : PRKAG1
Synonyms : PRKAG1; protein kinase, AMP-activated, gamma 1 non-catalytic subunit; 5-AMP-activated protein kinase subunit gamma-1; AMPK gamma1; AMPK gamma-1 chain; 5-AMP-activated protein kinase, gamma-1 subunit; AMPKG; MGC8666;
Gene ID : 5571
mRNA Refseq : NM_001206709
Protein Refseq : NP_001193638
MIM : 602742
UniProt ID : P54619
Chromosome Location : 12q12-q14
Pathway : AMPK signaling, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Energy Metabolism, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AM
Function : ADP binding; AMP binding; contributes_to AMP-activated protein kinase activity; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; nucleotide binding; protein bindi

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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