Recombinant Human PRMT5 2-637(C-term.) and MEP50 2-342 (C-term.) fused with N-terminal Flag (PRMT5), N-terminal His (MEP50) was expressed in HEK293.
Solution of purified recombinant protein in 50 mM Tris/HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM TCEP, 20% (v/v) glycerol.
Store at -80 centigrade, aliquot and snap-freeze after first use.