Recombinant Human PRMT5/WDR77(a.a: 2–end (both)) fused with N-terminal FLAG-tag (PRMT5) and N-terminal His-tag (WDR77) was expressed in FreeStyle 293-F cells.
Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 80 µg/ml Flag peptide, and 20% glycerol.
73 kDa (PRMT5), 37.5 kDa (MEP50)
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
At least 6 months at -80 centigrade. Avoid freeze/thaw cycles.