Recombinant Human PROC(Met1-Pro461) fused with His tag at C-terminal was expressed in NS0.
Predicted N Terminal:
Ala43 (mature and light chains) & Asp200 (heavy chain)
Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >25 pmol/min/µg.
Predicted Molecular Mass: 49 (mature), 31 (heavy), 18 (light) kDa;
SDS-PAGE: 60-64 kDa, 41-44 kDa and 22 kDa, reducing conditions.
<1.0 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 200 μg/mL in sterile, deionized water.