Recombinant Human PROC(Ala43-Pro461) fused with His tag at C-terminal was expressed in CHO. The isolated protein was activated by thrombin/thrombomodulin complex and further purified.
Predicted N Terminal:
Ala43 (light chain) & Leu212 (heavy chain)
Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >275 pmol/min/µg.
Predicted Molecular Mass: 19 (light chain), 29 (heavy chain) kDa;
SDS-PAGE: 22-25 kDa and 40-50 kDa, reducing conditions.
<1.0 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.