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Home > Products > Recombinant Proteins > Recombinant Human PSG6 protein, His-tagged

Recombinant Human PSG6 protein, His-tagged

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By Species
Human(7)
By Source
By Tag
His(3)
Cat.No.:
PSG6-201H
Product Overview:
Recombinant Human PSG6(Gln35-His424) fused with His tag at C-terminal was expressed in HEK293.
Description:
Pregnancy-specific glycoprotein 6 (PSG-6), also known as PSG10 and PSGGB is a secreted glycoprotein of the PSG family within the CEA (carcinoembryonic antigen) superfamily. Mature human PSG-6 is a 401 amino acid (aa) protein that consists of four tandem immunoglobulin-like domains followed by a 30 aa C-terminal tail. The N-terminal Ig-like domain contains an Arg-Gly-Asp (RGD) sequence which is a consensus integrin-binding motif found in many adhesion proteins. Alternative splicing generates an additional isoform with a substitution in the C-terminal tail. PSG-6 is predominantly expressed in placenta and more weakly in granulocytes. It induces monocyte production of the cytokines IL-6, IL-10, and TGF-beta 1.
Source:
HEK293
Species:
Human
Tag :
His
Predicted N Terminal:
No results obtained. Gln35 inferred from enzymatic pyroglutamate treatment revealing Val36
Form:
Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity:
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.9-4.5 μg/mL.
Molecular Mass:
Predicted Molecular Mass: 45 kDa
SDS-PAGE: 62-73 kDa, reducing conditions
Endotoxin:
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 500 μg/mL in PBS.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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