Recombinant Human PTBP2, fused with N-His tag, was expressed in E. coli.
RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA.
20mM Tris.Cl, 50mM NaCl, 50% Glycerol, pH8.0.
59.4kDa(including fusion tag)
546aa(including fusion tag)
WB, ELISA, IP, antibody production, protein array
Store at -80centigrade and avoid repeated freeze-thaw cycles