Recombinant Human PTGS2 protein, FLAG/His-tagged

• Cat.No.: PTGS2-32H
• Product Overview: Recombinant Human PTGS2(1-604 aa) fused with FLAG/His tag at C-terminal was expressed in Insect cells.

Specification

• Description: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
• Source: Insect cells
• Species: Human
• Tag: FLAG/His
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 µg/ml FLAG peptide, 300 µM diethyldithiocarbamate and 0.08% Triton X-100.
• Molecular Mass: 71 kDa
• AA Sequence: MLARALLLCAVLALSHTANPCCSHP CQNRGVCMSVGFDQYKCDCTRTGFY GENCSTPEFLTRIKLFLKPTPNTVH YILTHFKGFWNVVNNIPFLRNAIMS YVLTSRSHLIDSPPTYNADYGYKSW EAFSNLSYYTRALPPVPDDCPTPL GVKGKKQLPDSNEIVEKLLLRRKFI PDPQGSNMMFAFFAQHFTHQFFKTD HKRGPAFTNGLGHGVDLNHIYGETL ARQRKLRLFKDGKMKYQIIDGEMYP PTVKDTQAEMIYPPQVPEHLRFAVG QEVFGLVPGLMMYATIWLREHNRV CDVLKQEHPEWGDEQLFQTSRLILI GETIKIVIEDYVQHLSGYHFKLKFD PELLFNKQFQYQNRIAAEFNTLYH WHPLLPDTFQIHDQKYNYQQFIYNN SILLEHGITQFVESFTRQIAGRVAG GRNVPPAVQKVSQASIDQSRQMKYQ SFNEYRKRFMLKPYESFEELTGEKE MSAELEALYGDIDAVELYPALLVEK PRPDAIFGETMVEVGAPFSLKGLM GNVICSPAYWKPSTFGGEVGFQIIN TASIQSLICNNVKGCPFTSFSVPDP ELIKTVTINASSSRSGLDDINPTVL LKERSTELDYKDDDDKHHHHHH
• Purity: ≥90%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: At least 6 months at -80 centigrade. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
• Concentration: 0.33 mg/ml

Gene Information

• Gene Name: PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) [ Homo sapiens ]
• Official Symbol: PTGS2
• Synonyms: PTGS2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); prostaglandin G/H synthase 2; COX2; PHS II; PGH synthase 2; cyclooxygenase 2b; prostaglandin H2 synthase 2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
• Gene ID: 5743
• mRNA Refseq: NM_000963
• Protein Refseq: NP_000954
• MIM: 600262
• UniProt ID: P35354
• Chromosome Location: 1q25.2-q25.3
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Biological oxidations, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; COX reactions, organism-specific biosystem
• Function: enzyme binding; heme binding; heme binding; lipid binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; peroxidase activity; peroxidase activity; peroxidase activity; prostaglandin-endoperoxide synthase activity