Human parathyroid hormone(amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques
Human parathyroid hormone(hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts.
Liquid. In phosphate-buffered Saline(pH 7.4) containing 2 mM EDTA
Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 for this effect is less than or equal to 50 ng/ml.
9.55 kDa (85 aa), confirmed by MALDI-TOF
MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>95% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
1 mg/ml (determined by Bradford assay)