Active Recombinant Human PVR Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: PVR-653H
• Product Overview: Biotinylated Recombinant Human PVR Protein (Trp 21 - Asn 343) (AAH15542), fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc & Avi
• Predicted N Terminal: Trp 21
• Form: Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Tag at 2μg/mL (100 μL/well) can bind Biotinylated Human CD155, Fc Tag with a linear range of 0.02-0.15 μg/mL. Immobilized Human DNAM-1, Fc Tag at 2μg/mL (100 μL/well) can bind Biotinylated Human CD155, Fc Tag with a linear range of 0.02-0.08 μg/mL.
• Molecular Mass: Recombinant Human PVR Protein has a calculated MW of 63.5 kDa. As a result of Glycosylation, The protein migrates as 75-100 kDa under reducing (R) condition and 140-180 kDa under non-reducing (NR) condition on SDS-PAGE gel.
• Protein length: Trp 21 - Asn 343
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PVR poliovirus receptor [ Homo sapiens ]
• Official Symbol: PVR
• Synonyms: PVR; poliovirus receptor; PVS; CD155; HVED; Necl 5; NECL5; nectin like 5; Tage4; nectin-like 5; nectin-like protein 5; TAGE4; Necl-5; FLJ25946
• Gene ID: 5817
• mRNA Refseq: NM_001135768
• Protein Refseq: NP_001129240
• MIM: 173850
• UniProt ID: P15151
• Chromosome Location: 19q13.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Adherens junctions interactions, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem
• Function: cell adhesion molecule binding; protein binding; receptor activity