Recombinant Human RAC1 fused with His tag was expressed in E. coli.
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
20 mM Tris-HCl, 0.15 M NaCl, pH8.0
GSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMQAIKCVVVGDGAVGKTCLLISYTTNAFSGEYIPTVFDNYSA NVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTK LDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLL L
<1eu/ug as determined by the LAL method
~ 90% by SDS-PAGE analysis
For long term storage, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles.