Recombinant human RAD51B (383aa, Isoform-I, derived from BC030219) fused with T7-His-TEV cleavage site Tag at N-terminal and 11R tag fusion at C-terminal was expressed in E. coli.
1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
MASMTGGQQMGRGHHHHHHENLYFQGGEFGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYR GVHELLCMVSRACAPKMQTAYGIKAQRSADFSPAFLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMS ILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYFNTEEKLLLTSSKVHLYRELTCDEVLQRIESLEE EIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPVILTNQITTHLSGALASQAD LVSPADDLSLSEGTSGSSCVIAALGNTWSHSVNTRLILQYLDSERRQILIAKSPLAPFTSFVYTIKEEGLVLQET TFCSVTQAELNWAPEILPPQPPEQLGLQMCHHTQLIFLEESGGGGSPGRRRRRRRRRRR
1. May be used for in vitro RAD51B mediated double-stranded DNA repair pathway regulation study by intracellular delivery of this protein.2. May be used for RAD51B protein-protein interaction mapping.3. May be used as specific substrate protein for kinase and ubiquitin (Sumo pathway) ralted enzyme functional screening assays.4. As antigen for specific antibody production.
Keep at -80 centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.