Recombinant Human RBFOX2(aa 1-380) fused with His tag was expressed in E. coli.
RBFOX2 / RBM9 is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts.
20 mM Tris, pH 8.0, 500 mM NaCl, 50% glycerol
MEKKKMVTQG NQEPTTTPDA MVQPFTTIPF PPPPQNGIPTEYGVPHTQDY AGQTGEHNLT LYGSTQAHGE QSSNSPSTQNGSLTTEGGAQ TDGQQSQTQS SENSESKSTP KRLHVSNIPFRFRDPDLRQM FGQFGKILDV EIIFNERGSK GFGFVTFENSADADRAREKL HGTVVEGRKI EVNNATARVM TNKKMVTPYANGWKLSPVVG AVYGPELYAA SSFQADVSLG NDAAVPLSGRGGINTYIPLI SLPLVPGFPY PTAATTAAAF RGAHLRGRGRTVYGAVRAVP PTAIPAYPGV VYQDGFYGAD LYGGYAAYRYAQPATATAAT AAAAAAAAYS DGYGRVYTAD PYHALAPAASYGVGAVASLY RGGYSRFAPY
Long term: -80 centigrade; Short term: -20 centigrade. Avoid freeze-thaw cycles.