Recombinant Human RHOA protein, His-tagged
Cat.No. : | RHOA-32H |
Product Overview : | Recombinant Human RHOA fused with histidine tag in the amino-terminus was expressed in E. coli. The constitutively active form of human RhoA protein contains a glutamine to leucine substitution at residue 63. The common name for this mutant is RhoA(Q63L) or L63RhoA. The leucine substitution prevents endogenous and GAP-stimulated GTPase activity of RhoA, hence the protein is always in the active, GTP-bound, state. The corresponding mutation in Ras (Ras(Q61L)) behaves as an oncogenic mutation. |
- Specification
- Gene Information
- Related Products
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose and 0.1% dextran. |
Molecular Mass : | The recombinant protein is 25 kDa, consisting of the 22 kDa RhoA constitutively active protein plus a histidine tag in the amino-terminus. |
Purity : | 90% |
Applications : | RhoA effector and GAP identification and binding studies; Activation of RhoA effectors in vitro and in vivo; Stimulation of RhoA phenotype by microinjection into cells. |
Gene Name : | RHOA ras homolog family member A [ Homo sapiens ] |
Official Symbol : | RHOA |
Synonyms : | RHOA; ras homolog family member A; ARH12, ARHA, ras homolog gene family, member A; transforming protein RhoA; Rho12; RhoA; RHOH12; h12; oncogene RHO H12; rho cDNA clone 12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; ras homolog gene family, member A; ARHA; ARH12; RHO12; |
Gene ID : | 387 |
mRNA Refseq : | NM_001664 |
Protein Refseq : | NP_001655 |
MIM : | 165390 |
UniProt ID : | P61586 |
Chromosome Location : | 3p21.3 |
Pathway : | Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Arf6 downstream pathway, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; |
Function : | GTP binding; GTPase activity; myosin binding; nucleotide binding; protein binding; |
Products Types
◆ Recombinant Protein | ||
Rhoa-641M | Recombinant Mouse Rhoa Protein, MYC/DDK-tagged | +Inquiry |
RHOA-2655H | Recombinant Human RHOA Protein, His-tagged | +Inquiry |
RHOA-160H | Recombinant Human RHOA Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
RHOA-4694R | Recombinant Rat RHOA Protein, His (Fc)-Avi-tagged | +Inquiry |
RHOA-1888H | Recombinant Human RHOA Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
RHOA-601HCL | Recombinant Human RHOA cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0769 | RhoA Activation Colorimetric Assay Kit (96 assays) | +Inquiry |
Kit-0768 | RhoA Activation ColorimetricAssay Kit (24 assays) | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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