Recombinant Human RSPH9, fused with N-His tag, was expressed in E. coli.
Probable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk, which is attached to a subfiber of the outer doublet microtubule, and a bulbous head, which is attached to the stalk and appears to interact with the projections from the central pair of microtubules.
20mM Tris.Cl, 100mM NaCl, 50% Glycerol, pH8.0.
33.2kDa(including fusion tag)
291aa(including fusion tag)
WB, ELISA, IP, antibody production, protein array
Store at -80centigrade and avoid repeated freeze-thaw cycles