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Home > Products > Recombinant Proteins > Recombinant Human S100A9 protein

Recombinant Human S100A9 protein

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Human(13)
Mouse(6)
Rat(3)
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His(9)
N/A(1)
None(1)
StrepII(1)
Cat.No.:
S100A9-255H
Product Overview:
Recombinant Human S100A9(Thr2-Pro114) was expressed in E. coli.
Description:
S100A9, also known as MRP14 and Calgranulin B, is an approximately 14 kDa pro-inflammatory protein in the S100 family of calcium binding proteins. It is up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and is present at elevated concentrations in rheumatoid arthritis synovial fluid. Human S100A9 contains two EF-hand calcium binding motifs and shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively. S100A9 is a noncovalent homodimer that can also noncovalently heterodimerize with S100A8; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers. Human S100A9 can be phosphorylated at Thr113 near the C-terminus, a residue which is not conserved in the mouse protein. Human S100A9 can also be modified by S-glutathionylation and oxidation. The S100A8/A9 heterodimer binds to fatty acids such as arachidonic acid, while neither monomer does. S100A9 and the heterodimer promote neutrophil infiltration and degranulation at sites of inflammation and inflammatory cytokine production by monocytes. S100A9 also promotes cartilage matrix destruction in osteoarthritis as well as RAGE-dependent cardiac fibrosis and remodeling. In the brain, the S100A9 homodimer promotes neuronal cytotoxicity, although this is reduced by its binding to amyloid-beta peptide.
Source:
E. coli
Species:
Human
Predicted N Terminal:
Thr2
Form:
Lyophilized from a 0.2 μm filtered solution in Tris, TCEP and Trehalose.
Bio-activity:
Measured by its ability to induce CXCL1/KC secretion by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.8-4 μg/mL.
Molecular Mass:
Predicted Molecular Mass: 13 kDa
SDS-PAGE: 12 kDa, reducing conditions
Endotoxin:
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 400 μg/mL in water.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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