Recombinant Human SEMA4D(Met 22 - Arg 734) fused with a human IgG1 Fc tag at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Measured by its ability to promote the survival and differentiation of human peripheral blood monocytes.The ED50 for this effect is typically 2.0-6.3 μg/mL.
Has a calculated MW of 105.4 kDa. DTT-reduced Protein migrates as 120-140 kDa in SDS-PAGE due to glycosylation.
Met 22 - Arg 734
Less than 1.0 EU per μg of the Human Semaphorin 4D, Fc Tag by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).