Recombinant human SERPINB3 (AAB20405.1) (Asn 2-Pro 390) was expressed, with a polyhistidine tag at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 3mM DTT, 5%~8% trehalose and mannitol.
Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenice ptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.The IC50 is
The recombinant human SERPINB3 consists of 408 amino acids and predicts a molecular mass of 46.7 kDa. It migrates as an approximately 43 KDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>97 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.