Recombinant Human SESTD1, fused with N-His tag, was expressed in E. coli.
May act as the primary docking protein directing membrane turn over and assembly of the transient receptor potential channels TRPC4 and TRPC5. Binds phospholipids such as phosphatidylinositol monophosphates, phosphatidylinositol diphosphates (PIP2s) and phosphatidic acid, but not less polar lipids including phosphatidylcholine, phosphatidylserine, and phosphatidylinositol. The binding to PIP2s is calcium dependent. Might be involved in the plasma membrane localization of CTNNB1.
20mM Tris.Cl, 50mM NaCl, 50% Glycerol, pH8.0.
28kDa(including fusion tag)
240aa(including fusion tag)
WB, ELISA, IP, antibody production, protein array
Store at -80centigrade and avoid repeated freeze-thaw cycles