Recombinant Human SETD2(1345-1711 aa) fused with GST tag at N-terminal was expressed in E. coli.
SETD2 is a SET-domain containing histone methyltransferase, catalyzing the trimethylation of histone H3 at lysine 36. SETD2 is a candidate tumor suppressor protein and mutated in clear cell renal cell carcinoma.
In 50mM Tris pH 7.3, 300 mM NaCl, 4mM DTT, 1µM ZnCl2 and 25% glycerol.
SETD2, Recombinant Human GST-tagged is useful for histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 0.5 - 2.5 µg SETD2 per reaction with nucleosomes or recombinant histone H3 as a substrate is recommended.
Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.