Recombinant Human SETD2(1425-1717 aa) fused with GST tag at N-terminal was expressed in Insect cells.
As a member of the mixed lineage leukemia (MLL) family of histone methyltransferase, SETD2 specifically tri-methylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. The main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
1 year at -70 centigrade from the date of shipment
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.