Recombinant Human SETD6(1-473 aa) fused with GST tag at N-terminal was expressed in E. coli.
SETD6 is a SET-domain containing protein lysine methyltransferase, catalyzing the mono-methylation of RelA (p65, NFkB3) at lysine 310. Methylation of RelA attenuates RelA-dependant transcriptional pathways, including inflammatory responses in primary immune cells. SETD6 is downregulated in a diseases of hyper inflammation.
100 mM Tris pH 8.0, 10mM glutathione and 25% glycerol.
Recombinant SETD6, human is useful for RelA/p65 methylation experiments, enzyme kinetics and inhibitor screening. Use of 2-5 µg SETD6 per reaction with 1 µg recombinant RelA as a substrate is recommended.
Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.