Recombinant Human SETMAR, fused with N-His tag, was expressed in E. coli.
Histone methyltransferase that methylates "Lys-4" and "Lys-36" of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 "Lys-36". Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5"-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA.
20mM Tris.Cl, 50mM NaCl, 50% Glycerol, pH9.0.
42.1kDa(including fusion tag)
358aa(including fusion tag)
WB, ELISA, IP, antibody production, protein array
Store at -80centigrade and avoid repeated freeze-thaw cycles