Recombinant human SFRP4 (NP_003005.2) (Met1-VAL346) was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells.The ED50 for this effect is typically 2-20 μg/mL in the presence of 20 ng/mL of Wnt3a.
The recombinant human SFRP4 consists 336 amino acids and predicts a molecular mass of 39 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.