"SFRP4" Related Products

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Recombinant Human SFRP4 Protein, His-tagged

Cat.No.: SFRP4-247H
Product Overview: Recombinant human SFRP4 (NP_003005.2) (Met1-VAL346) was expressed with a polyhistidine tag at the C-terminus.
Source: CHO
Species: Human
Tag: His
Predicted N Terminal: Ala 22
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells.The ED50 for this effect is typically 2-20 μg/mL in the presence of 20 ng/mL of Wnt3a.
Molecular Mass: The recombinant human SFRP4 consists 336 amino acids and predicts a molecular mass of 39 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: SFRP4 secreted frizzled-related protein 4 [ Homo sapiens ]
Official Symbol: SFRP4
Synonyms: SFRP4; secreted frizzled-related protein 4; FRP 4; FRPHE; frpHE; sFRP-4; frizzled protein, human endometrium; FRP-4; MGC26498;
Gene ID: 6424
mRNA Refseq: NM_003014
Protein Refseq: NP_003005
MIM: 606570
UniProt ID: Q6FHJ7
Chromosome Location: 7p14.1
Pathway: Adipogenesis, organism-specific biosystem; Wnt Signaling Pathway, organism-specific biosystem; Wnt signaling pathway, organism-specific biosystem; Wnt signaling pathway, conserved biosystem;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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