Active Recombinant Human SFRP4 Protein, His-tagged

• Cat.No.: SFRP4-247H
• Product Overview: Recombinant human SFRP4 (NP_003005.2) (Met1-VAL346) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Source: CHO
• Species: Human
• Tag: His
• Predicted N Terminal: Ala 22
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells.The ED50 for this effect is typically 2-20 μg/mL in the presence of 20 ng/mL of Wnt3a.
• Molecular Mass: The recombinant human SFRP4 consists 336 amino acids and predicts a molecular mass of 39 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SFRP4 secreted frizzled-related protein 4 [ Homo sapiens ]
• Official Symbol: SFRP4
• Synonyms: SFRP4; secreted frizzled-related protein 4; FRP 4; FRPHE; frpHE; sFRP-4; frizzled protein, human endometrium; FRP-4; MGC26498
• Gene ID: 6424
• mRNA Refseq: NM_003014
• Protein Refseq: NP_003005
• MIM: 606570
• UniProt ID: Q6FHJ7
• Chromosome Location: 7p14.1
• Pathway: Adipogenesis, organism-specific biosystem; Wnt Signaling Pathway, organism-specific biosystem; Wnt signaling pathway, organism-specific biosystem; Wnt signaling pathway, conserved biosystem