Recombinant Human SIRPA(Glu 31 - Arg 370) fused with human IgG1 Fc tag at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Hs a calculated MW of 63.5 kDa. The reducing (R) protein migrates as 70-105 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of protein by the LAL method.
>95% as determined by SDS-PAGE.>95% as determined by SEC-HPLC.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).