Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human SIRPA protein, His-tagged

Recombinant Human SIRPA protein, His-tagged

   Share
"SIRPA" Related Products.
By Species
Chicken(1)
Human(19)
Mouse(13)
Rat(8)
By Source
+Expand -Collapse
By Tag
Fc(6)
His(9)
His & T7(2)
Cat.No.:
SIRPA-4849H
Product Overview:
Recombinant Human SIRPA(Met 1-Arg 370) fused with His tag at C-terminal was expressed in HEK293.
Description:
The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.
Source:
HEK293
Species:
Human
Tag :
His
Predicted N Terminal:
Glu 31
Form:
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Bio-activity:
Measured by its binding ability in a functional ELISA.
Immobilized human SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc (Cat:12283-H02H). The EC50 of human CD47-Fc (Cat:12283-H02H) is 10.1-23.5 ng/ml.
Molecular Mass:
The recombinant human SIRPA consists of 351 amino acids and predictes a molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSIRPA is approximately 45-50 kDa due to glycosylation.
Protein length:
Met 1-Arg 370
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method
Purity:
> 98 % as determined by SDS-PAGE
Stability:
Samples are stable for up to twelve months from date of receipt at -70 centigrade
Storage:
Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:
Reconstitute in PBS or others.
Shipping:
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.