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Home > Products > Recombinant Proteins > Recombinant Human SLAMF6 protein, His-tagged

Recombinant Human SLAMF6 protein, His-tagged

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By Species
Human(22)
Mouse(8)
Rat(1)
By Source
By Tag
Fc(1)
GST(2)
His(4)
Cat.No.:
SLAMF6-241H
Product Overview:
Recombinant Human SLAMF6(Gln22-Met226) fused with His tag at C-terminal was expressed in HEK293.
Description:
NTB-A, also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family. Mature human NTB-A consists of a 205 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set and one Ig-like C2-set domain, a 21 aa transmembrane segment, and an 84 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs). An alternatively spliced isoform is truncated in the cytoplasmic domain and lacks the two ITSMs. Within the ECD, human NTB-A shares 48% aa sequence identity with mouse and rat NTB-A. The ECD of human NTB-A shares 19%-34% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils. It interacts homophilically through weak associations between the Ig-V domains. NTB-A functions as an activating coreceptor on NK and T cells. Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells. Phosphorylation-dependent NTB-A association with SAP is required for full production of IFN-gamma by NK cells. This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM. NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators. In B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity. In addition, NTB-A binds to the influenza virus hemagglutinin (HA) protein which co-stimulates NK cell activation. The Vpu protein encoded by HIV-1 interferes with NTB-A glycosylation and cell surface expression.
Source:
HEK293
Species:
Human
Tag :
His
Predicted N Terminal:
No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Ser23
Form:
Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity:
Measured by its binding ability in a functional ELISA. When Recombinant Human NTB-A/SLAMF6 is coated at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human NTB-A/SLAMF6 Fc Chimera that produces 50% optimal binding response is 0.6-3 μg/mL.
Molecular Mass:
Predicted Molecular Mass: 24 kDa
SDS-PAGE: 34-42 kDa, reducing conditions
Endotoxin:
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
6 months, -70 centigrade under sterile conditions after opening.
Reconstitution:
Reconstitute at 500 μg/mL in PBS.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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