Recombinant Human SMUG1 was expressed in E. coli.
10 mM Bis-Tris-Propane-HCl, 10 mM MgCl2, 1 mM DTT, pH 7 @ 25 centigrade.
Theoretical: 29861 daltons
One unit is defined as the amount of enzyme required to excize 1 pmol of deoxyuracil from a 34 mer oligonucleotide duplex containing a single dU site in a total reaction volume of 10 µl in 1 hour at 37 centigrade.
Store at -20 centigrade