Recombinant Human SMYD3 protein, GST-tagged

• Cat.No.: SMYD3-263H
• Product Overview: Recombinant Human SMYD3 fused with GST tag at N-terminal was expressed in E. coli.

Specification

• Description: SMYD3 is a protein lysine methyltransferase that has been shown to catalyze mono-, di-, and tri-methylation of histone H4 at lysine 5 by multiple independent approaches, including mass spectrometry. SMYD3 levels are up-regulated in a large number of tumors, including liver, breast and rectal carcinomas. SMYD3 overexpression promotes the proliferation of cancer cells, while its depletion slows expansion.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Recombinant GST-SMYD3 (1 µg/µl) in 100mM Tris pH 8.0, 10 mM glutathione and 10% glycerol.
• Molecular Mass: 68 kDa
• Applications: Recombinant SMYD3, human is useful for histone H4 methylation experiments, enzyme kinetics and inhibitor screening. Use of 1-3 µg SMYD3 per reaction with recombinant histone H4 as a substrate is recommended.
• Storage: Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
• Concentration: 1 µg/µl

Gene Information

• Gene Name: SMYD3 SET and MYND domain containing 3 [ Homo sapiens ]
• Official Symbol: SMYD3
• Synonyms: SMYD3; SET and MYND domain containing 3; zinc finger, MYND domain containing 1 , ZMYND1, ZNFN3A1; SET and MYND domain-containing protein 3; KMT3E; bA74P14.1 (novel protein); zinc finger, MYND domain containing 1; zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; ZMYND1; ZNFN3A1; bA74P14.1; FLJ21080; MGC104324
• Gene ID: 64754
• mRNA Refseq: NM_001167740
• Protein Refseq: NP_001161212
• MIM: 608783
• UniProt ID: Q9H7B4
• Chromosome Location: 1q44