Recombinant Human SMYD3 fused with GST tag at N-terminal was expressed in E. coli.
SMYD3 is a protein lysine methyltransferase that has been shown to catalyze mono-, di-, and tri-methylation of histone H4 at lysine 5 by multiple independent approaches, including mass spectrometry. SMYD3 levels are up-regulated in a large number of tumors, including liver, breast and rectal carcinomas. SMYD3 overexpression promotes the proliferation of cancer cells, while its depletion slows expansion.
Recombinant GST-SMYD3 (1 µg/µl) in 100mM Tris pH 8.0, 10 mM glutathione and 10% glycerol.
Recombinant SMYD3, human is useful for histone H4 methylation experiments, enzyme kinetics and inhibitor screening. Use of 1-3 µg SMYD3 per reaction with recombinant histone H4 as a substrate is recommended.
Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.