Recombinant Human SNRNP70 was expressed in E. coli.
U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA and 0.02% SDS.
Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein (lacking 66 internal amino acids outside the known epitope-containing areas) fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa (U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis).
Greater than 90% as determined by SDS-PAGE.
Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera (Mixed Connective Tissue Disease).
Store at 4 centigrade if entire vial will be used within 2-4 weeks.
Store, frozen at -20 centigrade for longer periods of time.
Avoid multiple freeze-thaw cycles.