"SPHK1" Related Products

Recombinant Human SPHK1 protein, Flag-tagged, Biotinylated

Cat.No.: SPHK1-77H
Product Overview: Recombinant Human SPHK1 [1-384(end) amino acids of accession number NP_001136074.1] fused with DYKDDDDK tag at N-terminal was expressed in insect cell and biotinylated.
Source: Insect cell
Species: Human
Tag: Flag
Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Molecular Mass: 46 kDa
Purity: >90% as determined by SDS-PAGE and CBB staining
Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens ]
Official Symbol: SPHK1
Synonyms: SPHK1; sphingosine kinase 1; SPHK; SK 1; SPK 1;
Gene ID: 8877
mRNA Refseq: NM_001142601
Protein Refseq: NP_001136073
MIM: 603730
UniProt ID: Q9NYA1
Chromosome Location: 17q25.2
Pathway: Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chaperonin-mediated protein folding, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Fc-epsilon receptor I signaling in mast cells, organism-specific biosystem;
Function: ATP binding; D-erythro-sphingosine kinase activity; DNA binding; calmodulin binding; diacylglycerol kinase activity; magnesium ion binding; magnesium ion binding; nucleotide binding; protein binding; protein phosphatase 2A binding; sphinganine kinase activity; transferase activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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