Recombinant Human SPHK2 [1-618(end) amino acids of accession number NP_001191089.1] fused with DYKDDDDK tag at N-terminal was expressed in insect cell and biotinylated.
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
>80% as determined by SDS-PAGE and CBB staining
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.