Recombinant Human SRSF1(1-248aa) fused with His tag at C-terminal was expressed in Insect cells.
SRSF1, also known as Serine/arginine-rich splicing factor 1, is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. This protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. In addition to being involved in the splicing process, it also mediates post-splicing activities, such as mRNA nuclear export and translation.
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 100mM KCl, 1mM DTT, 0.2mM EDTA, 40% Glycerol
28.5kDa (254aa) 28-40KDa (SDS-PAGE under reducing conditions.)
MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPRSGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKFRSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRTHH HHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
> 95% by SDS - PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.25mg/ml (determined by Bradford Assay)