Recombinant Human ST3GAL2 /SIAT4B Protein (rh ST3GAL2 / SIAT4B) Pro 52 - Asn 350 (Accession # AAH36777) was produced in human 293 cells (HEK293).
Predicted N Terminal:
Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 7.5, 10% Trehalose.
Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to bovine asialofetuin. The specific activity is >750 pmol/min/ug.
Human ST3GAL2, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 35.2 kDa. The reducing (R) protein migrates as 36 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>92% as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.