Active Recombinant Human ST3GAL2 Protein, His-tagged

• Cat.No.: ST3GAL2-577H
• Product Overview: Recombinant Human ST3GAL2 /SIAT4B Protein (rh ST3GAL2 / SIAT4B) Pro 52 - Asn 350 (Accession # AAH36777) was produced in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Pro 52
• Form: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 7.5, 10% Trehalose.
• Bio-activity: Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to bovine asialofetuin. The specific activity is >750 pmol/min/ug.
• Molecular Mass: Human ST3GAL2, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 35.2 kDa. The reducing (R) protein migrates as 36 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ST3GAL2 ST3 beta-galactoside alpha-2,3-sialyltransferase 2 [ Homo sapiens ]
• Official Symbol: ST3GAL2
• Synonyms: ST3GAL2; ST3 beta-galactoside alpha-2,3-sialyltransferase 2; sialyltransferase 4B (beta galactosidase alpha 2,3 sialytransferase) , SIAT4B; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2; ST3GalA.2; ST3GALII; SIAT4-B; ST3Gal II; alpha 2,3-ST 2; beta-galactoside alpha-2,3-sialytransferase; beta-galactoside alpha-2,3-sialyltransferase 2; Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; sialyltransferase 4B (beta-galactosidase alpha-2,3-sialytransferase); SIAT4B; Gal-NAc6S
• Gene ID: 6483
• mRNA Refseq: NM_006927
• Protein Refseq: NP_008858
• MIM: 607188
• UniProt ID: Q16842
• Chromosome Location: 16q22.3
• Pathway: Glycosaminoglycan biosynthesis - keratan sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - keratan sulfate, conserved biosystem; Glycosphingolipid biosynthesis - ganglio series, organism-specific biosystem; Glycosphingolipid biosynthesis - ganglio series, conserved biosystem; Glycosphingolipid biosynthesis - globo series, organism-specific biosystem; Glycosphingolipid biosynthesis - globo series, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: beta-galactoside (CMP) alpha-2,3-sialyltransferase activity